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Human mitochondrial tRNAMet is exported to the cytoplasm and associates with the Argonaute 2 protein

机译:人线粒体tRNAMet被输出到细胞质并与Argonaute 2蛋白相关

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摘要

Argonaute (Ago) proteins are the effector proteins of RNA interference (RNAi) and related silencing mechanisms that are mediated by small RNAs. Ago proteins bind directly to microRNAs (miRNAs) and to short interfering RNAs and are the core protein components of RNA induced silencing complexes (RISCs) and microRNPs (miRNPs). Here we report that an ~70-nt RNA associates specifically with immunopurified Ago2 expressed in human 293 cells. By directional cloning we identified this RNA as the mitochondrial tRNAMet (mt tRNAMet). Various exported (mt) tRNAs were detected in the cytosol of 293 cells, but Ago2 was found selectively bound to (mt) tRNAMet. The association in the cytosol of exported (mt) tRNAMet with Ago2 complements genetic and microscopic data that link mitochondria with RNAi-related components and events.
机译:Argonaute(Ago)蛋白是RNA干扰(RNAi)和由小RNA介导的相关沉默机制的效应蛋白。前蛋白直接与微小RNA(miRNA)和短干扰RNA结合,并且是RNA诱导沉默复合物(RISC)和microRNP(miRNP)的核心蛋白成分。在这里,我们报告〜70 nt RNA专门与人类293细胞中表达的免疫纯化Ago2相关联。通过定向克隆,我们将该RNA鉴定为线粒体tRNAMet(mt tRNAMet)。在293细胞的细胞质中检测到各种输出的(mt)tRNA,但发现Ago2与(mt)tRNAMet选择性结合。出口(mt)tRNAMet与Ago2的胞浆中的关联补充了将线粒体与RNAi相关成分和事件联系起来的遗传和微观数据。

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